Measurement of Reactive Oxygen Species, Reactive Nitrogen Species, and Redox-Dependent Signaling in the Cardiovascular System

Published: July 14, 2016

  • Reactive oxygen species (ROS) and reactive nitrogen species (RNS) contribute to cardiovascular disease (CVD) initiation, progression, and severity.
  • Reliable measures of ROS/RNS are difficult to achieve due to short half-lives and high reactivity, but are critical for consistency across studies.
  • This statement aims to review current methods for measuring ROS, RNS, and their secondary products; to discuss the strengths of limitations of specific approaches; and to provide guidance for the reliable and reproducible measurement of reactive species.